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Vahid Ebrahimi*, J Tang*, KY Han (2021) "Incoherent superposition of polychromatic light enables single-shot nondiffracting light-sheet microscopy" Opt. Express, 29, 32691-32699.

Jinhan Ren, KY Han (2021) "2.5D microscopy with polarization independent SLM for enhanced detection efficiency and aberration correction" Opt. Express, 29, 27530-27541.

Chun Hung Weng, J Tang, KY Han (2021) "Optimizing the performance of multiline-scanning confocal microscopy" J. Phys. D: Appl. Phys., 54, 105401.

Jinhan Ren, KY Han (2021) "2.5D microscopy: Fast, high-throughput imaging via volumetric projection for quantitative subcellular analysis" ACS Photonics, 8, 933-942.


Benjamin Croop, J Tang, KY Han (2020) "Single-shot, shadowless total internal reflection fluorescence microscopy via annular fiber bundle" Opt. Lett., 45, 6470-6473.

Jialei Tang, KY Han (2020) "Instantaneous non-diffracting light-sheet generation by controlling spatial coherence", Opt. Comm. 474, 126154.


Jialei Tang*, Jinhan Ren*, KY Han (2019) "Fluorescence imaging with tailored light" Nanophotonics, 8, 2111-2128.

JC Lee, Y Ma, KY Han, T Ha (2019) "Accurate background subtraction in STED nanoscopy by polarization switching" ACS Photonics 6, 1789-1797.

Jialei Tang, KY Han (2019) "Low-photobleaching line-scanning confocal microscopy using dual inclined beams" J. Biophotonics, e201900075.

J Kim, KY Han, N Khanna, T Ha, AS Belmont (2019) "Nuclear speckle fusion via long-range directional motion regulates speckle morphology after transcriptional inhibition" J. Cell. Sci., 132, jcs226563.

Selected as the journal cover; See also an interview with First Person

Jialei Tang, CH Weng, JB Oleske, KY Han (2019) "A guide to Build a highly inclined swept tile microscope for extended field-of-view single-molecule imaging" JoVE, e59360.

Benjamin Croop, KY Han (2019) "Facile single-molecule pull-down assay for analysis of endogenous proteins" Phys. Biol., 16, 035002.

Special Issue on Development and Applications of Single-Molecule & Super-Resolution Imaging

Benjamin Croop*, C Zhang*, Y Lim*, RM Gelfand, KY Han (2019) "Recent advancement of light-based single-molecule approaches for studying biomolecules" WIREs Syst. Biol. Med., 11, e1445.


Jialei Tang, KY Han (2018) "Extended field-of-view single-molecule imaging by highly inclined swept illumination" Optica, 5, 1063-1069. [bioRxiv 311753]

KY Han, T Ha (2018) "Measuring molecular mass with light" Nature Photonics (News & Views,) 12, 380–381.

Ian Khaw*, Benjamin Croop*, J Tang, A Möhl, U Fuchs, KY Han (2018) "Flat-field illumination for quantitative fluorescence imaging" Opt. Express, 26, 15276-15288.

B Hua, Y Wang, S Park, KY Han, D Singh, JH Kim, W Cheng, T Ha (2018) "Single-molecule centroid localization algorithm improves the accuracy of fluorescence binding assays" Biochemistry, 57, 1572–1576.


Goun Je, Benjamin Croop, S Basu, J Tang, KY Han*, YS Kim* (2017) "Endogenous alpha-synuclein protein analysis from human brain tissues using single-molecule pull-down assay" Anal. Chem., 89, 13044-13048.

Jialei Tang, Y Sun, S Pang, KY Han (2017) "Spatially encoded fast single-molecule fluorescence spectroscopy with full field-of-view" Sci. Rep. 7, 10945.

I Masuda, T Igarasgi, R Sakaguchi, R Nitharwal, R Takase, KY Han, BJ Leslie, C Liu, H Gamper, T Ha, S Sanyal, YM Hou (2017) "A genetically encoded fluorescent tRNA is active in live-cell protein synthesis" Nucleic Acids Res., 45, 4081-4093.

Prior to CREOL

"A chemical controller of SNARE-driven membrane fusion that primes vesicles for Ca2+-triggered millisecond exocytosis"
Heo P, Yang Y, Han KY, Kong B, Shin J, Jung Y, Jeong C, Shin JH, Shin YK, Ha T, Kweon DH
J. Am. Chem. Soc. 138, 4512-4521 (2016)

"RESOLFT nanoscopy with photoswitchable organic fluorophores"
Kwon J, Hwang J, Park J, Han GR, Han KY*, Kim SK*
Sci. Reports 5, 17804 (2015)

"Tandem Spinach array for mRNA imaging in living bacterial cells"
Zhang J, Fei J, Leslie BJ, Han KY, Kuhlman TE, Ha T
Sci. Reports 5, 17295 (2015)

"Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser"
Han KY, Ha T
Opt. Lett. 40, 2653-2656 (2015)

"An Improved surface passivation method for single-molecule studies"
Hua B, Han KY, ..., Ha T
Nature Methods 11, 1233-1236 (2014)

"Understanding the photophysics of the Spinach-DFHBI RNA aptamer-fluorogen complex to improve live cell RNA imaging"
Han KY, Leslie BJ, Fei J, Zhang J, Ha T
J. Am. Chem. Soc. 135, 19033-19038 (2013)

"Preparation of non-aggregated fluorescent nanodiamonds (FNDs) by non-covalent coating with a block copolymer and proteins for enhancement of intracellular uptake"
Lee JW, Lee S, Jang S, Han KY, Kim Y, Hyun J, Kim SK, Lee Y
Molecular BioSystems 9, 1004-1011 (2013)

"Dark state photophysics of nitrogen–vacancy centres in diamond"
Han KY*, Wildanger D*, Rittweger E, Meijer J, Pezzagna S, Hell SW, Eggeling C
New J. Phys. 14, 123002 (2012)

"Sharper low-power STED nanoscopy by time gating"
Vicidomini G*, Moneron G*, Han KY*, Westphal V, Ta T, Reuss M, Engelhardt J, Eggeling C, Hell SW
Nature Methods 8, 571-573 (2011)

"Metastable dark states enable ground state depletion microscopy of nitrogen vacancy centers in diamond with diffraction-unlimited resolution"
Han KY, Kim SK, Eggeling C, Hell SW
Nano Lett. 10, 3199-3203 (2010)

"Three-dimensional stimulated emission depletion microscopy of nitrogen-vacancy centers in diamond using continuous-wave light"
Han KY, Willig KI, Rittweger E, Jelezko F, Eggeling C, Hell SW
Nano Lett. 9, 3323-3329 (2009)

"STED microscopy reveals crystal colour centres with nanometric resolution"
Rittweger E*, Han KY*, Irvine SE*, Eggeling C, Hell SW
Nature Photonics 3,144-147 (2009)


"STED microscopy with pulsed excitation, continuous stimulation, and gated registration of spontaneously emitted fluorescence light"
Hell SW, Eggeling C, Engelhardt J, Han KY, Moneron G, Matthias R, Vicidomini G, Westphal V.
World patent WO 2012069076 A1; US patent US 20130256564 A1

Commercially available from Leica Microsystems (TCS SP8 STED 3x)

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